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RFFL Antikörper (AA 1-363)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RFFL in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human.
Rockland
Produktnummer ABIN129535
Hersteller Produkt- Nr.: 200-401-932

Kurzübersicht für RFFL Antikörper (AA 1-363) (ABIN129535)

Target

Alle RFFL Antikörper anzeigen
RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Reaktivität

  • 41
  • 21
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
Human

Wirt

  • 38
  • 3
Kaninchen

Klonalität

  • 39
  • 2
Polyklonal

Konjugat

  • 16
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser RFFL Antikörper ist unkonjugiert

Applikation

  • 33
  • 16
  • 13
  • 13
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 15
    • 9
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 1-363

    Hersteller Produkt- Nr.

    200-401-932

    Hersteller

    Rockland

    Verwendungszweck

    RFFL Antibody

    Kreuzreaktivität (Details)

    This protein A purified antibody is directed against human RFFL protein.

    Produktmerkmale

    Synonyms: rabbit anti-RFFL antibody, Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like

    Aufreinigung

    The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.

    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-363 of human RFFL protein.

    Immunogen Type: Recombinant Protein

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:500 - 1:3,000

    Application Note: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RFFL protein by western blotting in the appropriate tissue or cell lysate or extract.  Isoforms 2 and 3 are 39.7 and 36.6 kDa, respectively and should also cross react with this antibody.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:4,000 - 1:20,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    5.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

    Andere Bezeichnung

    RFFL

    Hintergrund

    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). RFFL, also known as Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kB activation.  RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein. Anti-RFFL Antibody is useful for researcher interested in Cancer, Immunology, transcription, and TNF-alpha/NF-kB research.

    Gen-ID

    117584, 17432433

    UniProt

    Q8WZ73
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