CD97 Antikörper (AA 1-512)

Produktnummer ABIN129531

Hersteller Produkt- Nr.: 200-401-908

Dieses Anti-CD97-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CD97 in WB, ELISA, IF und FM. Geeignet für Human und Schimpanse.

Kurzübersicht für CD97 Antikörper (AA 1-512) (ABIN129531)

Target: CD97

Reaktivität: Human, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD97 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-CD97 Antikörper

Bindungsspezifität: AA 1-512

Hersteller Produkt- Nr.: 200-401-908

Hersteller: Rockland

Verwendungszweck: CD97 Antibody

Kreuzreaktivität (Details): This Protein A purified antibody is directed against the mouse CD97 protein.

Produktmerkmale: Synonyms: rabbit anti-CD97 Antibody, CD97 antigen antibody, CD97 Molecule antibody, Leukocyte antigen CD97 antibody, CD97 antigen subunit alpha, CD97 antigen subunit beta

Aufreinigung: The product was protein A purified from monospecific antiserum.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This Protein A purified antibody has been tested for use in immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 81 kDa in size corresponding to isoform 1 of CD97 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu CD97

Target: CD97

Andere Bezeichnung: Cd97

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Anti-CD97 is ideal for researchers interested in cancer, angiogenesis, and inflammation.

Gen-ID: 26364, 251823835

UniProt: Q9Z0M6