BUB1 Antikörper (AA 281-419)

Produktnummer ABIN129514

Produktdetails anti-BUB1 Antikörper

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Bindungsspezifität: AA 281-419

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BUB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 281-419 of human BUB1 protein.
Immunogentype:Recombinant

Klon: 14H5

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 160 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control.  For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8 OR 100% methanol at -20° C.  Permeabilize fixed cells with 0.5% Triton X-100.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Andere Bezeichnung: BUB1 (BUB1 Produkte)

Synonyme: bub1a antikoerper, bub1l antikoerper, hbub1 antikoerper, xbub1 antikoerper, BUB1A antikoerper, BUB1L antikoerper, hBUB1 antikoerper, AL022991 antikoerper, Bub1a antikoerper, C80208 antikoerper, D2Xrf87 antikoerper, BUB1 mitotic checkpoint serine/threonine kinase antikoerper, BUB1, mitotic checkpoint serine/threonine kinase antikoerper, BUB1 antikoerper, bub1 antikoerper, Bub1 antikoerper

Hintergrund: BUB1 (also called Mitotic checkpoint serine/threonine-protein kinase BUB1and BUB1A) is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating BUB3, a member of the miotic checkpoint complex, and activating the spindle checkpoint.  Mutations in this gene have been associated with aneuploidy and several forms of cancer.   BUB1 is autophosphorylated when cells enter mitosis.  This protein is localized to the nucleus in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. BUB1 is highly expressed in testis and thymus, less in colon, spleen, lung and small intestine.  Expression is associated with cells/tissues with a high mitotic index.
Synonyms: Budding uninhibited by benzimidazoles 1 homolog antibody, hBUB1 antibody, Mitotic checkpoint serine/threonine protein kinase BUB1 antibody

Gen-ID: 699

NCBI Accession: NP_004327

UniProt: Q53QE4