MAD2L1 Antikörper

Produktnummer ABIN129510

Hersteller Produkt- Nr.: 200-301-461

Dieses Anti-MAD2L1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MAD2L1 in WB, ELISA und IP. Geeignet für Human.

Kurzübersicht für MAD2L1 Antikörper (ABIN129510)

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAD2L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: 17D10

Produktdetails anti-MAD2L1 Antikörper

Hersteller Produkt- Nr.: 200-301-461

Hersteller: Rockland

Verwendungszweck: MAD2L1 Antibody

Kreuzreaktivität (Details): This Protein A purified antibody is directed against human MAD2L1 protein.

Produktmerkmale: Synonyms: mouse anti-MAD2L1 antibody, Mitotic arrest deficient yeast homolog like 1 antibody, Mitotic spindle assembly checkpoint protein antibody

Aufreinigung: The product was purified from tissue culture supernatant by chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation and by western blot.  This antibody is not applicable for immunofluorescence microscopy.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MAD2L1

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Andere Bezeichnung: MAD2L1

Hintergrund: Background: MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization.  A MAD2 pseudogene has been mapped to chromosome 14.

Gen-ID: 4085, 4505067

UniProt: Q13257