Cathepsin B Antikörper

Produktnummer ABIN127046

Der Kaninchen Polyklonal anti-Cathepsin B Antikörper wird verwendet zum Nachweis von Cathepsin B in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für Cathepsin B Antikörper (ABIN127046)

Target: Cathepsin B (CTSB)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cathepsin B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Cathepsin B Antikörper

Spezifität: The purified antibody detects both the proform (37-43 kDa) and the mature form (25 kDa) of Cathepsin B.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse and Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 322 of human cathepsins B

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Details zu Cathepsin B

Target: Cathepsin B (CTSB)

Andere Bezeichnung: Cathepsin B

Hintergrund: Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form (25 and 5 kDa subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.Synonyms: APP secretase, APPS, CPSB, CTSB, Cathepsin B1

Gen-ID: 1508

NCBI Accession: NP_001899

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades