PRKDC Antikörper

Produktnummer ABIN127020

Der Kaninchen Polyklonal anti-PRKDC Antikörper (ABIN127020) detektiert spezifisch PRKDC in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Hamster.

Kurzübersicht für PRKDC Antikörper (ABIN127020)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reaktivität: Human, Maus, Ratte, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKDC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PRKDC Antikörper

Spezifität: The antibody detects a >350,000 kDa and a 180,000 kDa protein (believed to be a processed form of the catalytic subunit), corresponding to the expected molecular weight of DNA-PKcs.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 4088 of human DNA- PK.

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5-4 μg/mL. HeLa cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Details zu PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Andere Bezeichnung: DNA-PKcs / PRKDC / XRCC7

Hintergrund: DNA-dependent protein kinase (DNA-PK) is made up of 3 subunits, 2 Ku proteins and a large catalytic subunit (DNA-PKcs). DNA-PKcs falls into the phosphatidyl-inositol 3-kinase superfamily although it does have serine/threonine protein kinase activity. This subunit migrates on SDS-PAGE gels as an intact and smaller processed form. In vitro, DNA-PK phosphorylates several transcription factors and other DNA-binding proteins and is believed to play a role in DNA damage recognition, repair and transcription. There is evidence to suggest that DNA-PKcs may be a critical target for proteolysis by a caspase in apoptosis.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460

Gen-ID: 5591

NCBI Accession: NP_001075109

UniProt: P78527

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response