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PLSCR1 Antikörper (phospholipid Scramblase 1) N-Term Primary Antibody

PLSCR1 Reaktivität: Human WB Wirt: Maus Monoclonal 1-00E-009 unconjugated
Produktnummer ABIN126886
$663.30
Zzgl. Versandkosten $45.00
0.1 mg
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Available
  • Target Alle PLSCR1 Antikörper anzeigen
    PLSCR1
    Bindungsspezifität
    • 9
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 30
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 22
    • 10
    Maus
    Klonalität
    • 25
    • 7
    Monoklonal
    Konjugat
    • 25
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PLSCR1 Antikörper ist unkonjugiert
    Applikation
    • 23
    • 10
    • 9
    • 7
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Spezifität
    This antibody specifically recognizes the N-terminus of scramblase-1 at 35 kDa.
    Produktmerkmale
    Synonyms: Phospholipid scramblase 1, PL scramblase 1, MmTRA1b, Ca(2+)-dependent phospholipidscramblase 1, Erythrocyte phospholipid scramblase
    Aufreinigung
    Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
    Bestandteile
    incl. positive Control
    Immunogen
    Synthetic peptide conjugated to KLH.
    Klon
    1-00E-009
    Isotyp
    IgG1
  • Applikationshinweise
    Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    Positive Control Cell LysateHepG2 untreatedSize Sufficient: 20 mini-gel lanes. Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for Immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore with 1 mL H2O (15 min, RT).
    Buffer
    1 mLPBS containing 0.09 % Sodium Azide as preservative, PEG and Sucrose.
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C/-80 °C
    Informationen zur Lagerung
    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Haltbarkeit
    12 months
  • Target
    PLSCR1
    Abstract
    PLSCR1 Produkte
    Synonyme
    mmtra1b, MGC84969, PLSCR1, MGC148322, MMTRA1B, MmTRA1a, MmTRA1b, Nor1, Tra1, Tra1a, Tra1b, Tras1, Tras2, PLSCR2, phospholipid scramblase 1 L homeolog, phospholipid scramblase 1, plscr1.L, PLS1, PVX_111580, PLSCR1, plscr1, Plscr1, LOC100712949, PLSCR2, LOC100341366, LOC611500
    Hintergrund
    Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases.Synonyms: Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1, Phospholipid scramblase 1
    Gen-ID
    5359
    UniProt
    O15162
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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