Phosphoserine Antikörper (phosphorylated)

Produktnummer ABIN126864

Der Maus Monoklonal anti-Phosphoserine Antikörper (Klon 7F12) (ABIN126864) detektiert spezifisch Phosphoserine in WB, IP und EIA.

Kurzübersicht für Phosphoserine Antikörper (phosphorylated) (ABIN126864)

Target: Phosphoserine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphoserine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Klon: 7F12

Produktdetails anti-Phosphoserine Antikörper

Bindungsspezifität: phosphorylated

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat and Dog.

Aufreinigung: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILKOR CASEIN FOR BLOCKING! ELISA: 0.05 μg/mL. Immunoprecipitation: 1-10 μg per 10^6 pervanadate-treated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive control: pSer / pThr Molecular Weight MarkerFormulation:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL H2O (15 min, RT)

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Phosphoserine

Target: Phosphoserine

Substanzklasse: Amino Acid

Hintergrund: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.