c-FOS Antikörper (N-Term)
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- Target Alle c-FOS (c-Fos) Antikörper anzeigen
- c-FOS (c-Fos) (c-Fos)
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Bindungsspezifität
- N-Term
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Reaktivität
- Human, Maus, Ratte, Hund
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser c-FOS Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Spezifität
- This antibody specifically interacts with the N-terminus of c-Fos. The antibody is suitable for Western blot and ELISA applications. It can be used for the detection of cellular Fos levels that might dramatically change during signal transduction. It is an important tool in combination with mab Fos-34E4 (phospho-Ser374) for studying fos expression and phosphorylation.
- Produktmerkmale
- Synonyms: FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatoryprotein 7
- Aufreinigung
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
- Bestandteile
- incl. positive Control
- Immunogen
- Synthetic peptide conjugated to KLH.
- Klon
- 8B5
- Isotyp
- IgG1
- Top Product
- Discover our top product c-Fos Primärantikörper
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- Applikationshinweise
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Restore with 1 mL H2O (15 min, RT).
- Buffer
- 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C/-80 °C
- Informationen zur Lagerung
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch - Haltbarkeit
- 12 months
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- Target
- c-FOS (c-Fos) (c-Fos)
- Andere Bezeichnung
- C-Fos (c-Fos Produkte)
- Synonyme
- AP-1 antikoerper, C-FOS antikoerper, p55 antikoerper, c-fos antikoerper, D12Rfj1 antikoerper, cFos antikoerper, cFOS antikoerper, fos antikoerper, Fos proto-oncogene, AP-1 transcription factor subunit antikoerper, FBJ osteosarcoma oncogene antikoerper, proto-oncogene c-Fos-like antikoerper, FOS antikoerper, Fos antikoerper, LOC101078177 antikoerper
- Hintergrund
- The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.Synonyms: Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos
- Gen-ID
- 2353
- UniProt
- P01100
- Pathways
- S100 Proteine
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