c-FOS Antikörper (N-Term)

Produktnummer ABIN126801

Dieses Anti-c-FOS-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von c-FOS in WB, IHC (fro) und EIA. Geeignet für Human, Ratte, Maus und Hund.

Kurzübersicht für c-FOS Antikörper (N-Term) (ABIN126801)

Target: c-FOS (c-Fos) (c-Fos)

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser c-FOS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 8B5

Produktdetails anti-c-FOS Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody specifically interacts with the N-terminus of c-Fos. The antibody is suitable for Western blot and ELISA applications. It can be used for the detection of cellular Fos levels that might dramatically change during signal transduction. It is an important tool in combination with mab Fos-34E4 (phospho-Ser374) for studying fos expression and phosphorylation.

Produktmerkmale: Synonyms: FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatoryprotein 7

Aufreinigung: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch

Haltbarkeit: 12 months

Details zu c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Andere Bezeichnung: C-Fos

Hintergrund: The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.Synonyms: Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos

Gen-ID: 2353

UniProt: P01100

Pathways: S100 Proteine