c-FOS Antikörper (pSer374)

Produktnummer ABIN126800

Der Maus Monoklonal Anti-c-FOS-Antikörper wurde für WB, IHC (fro) und EIA validiert. Er ist geeignet, c-FOS in Proben von Human und Hund zu detektieren.

Kurzübersicht für c-FOS Antikörper (pSer374) (ABIN126800)

Target: c-FOS (c-Fos) (c-Fos)

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser c-FOS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 3-40E-005

Produktdetails anti-c-FOS Antikörper

Bindungsspezifität: pSer374

Spezifität: This antibody specifically interacts with c-Fos phosphorylated at serine 374.

Produktmerkmale: Synonyms: FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatoryprotein 7

Aufreinigung: Size exclusion chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch

Haltbarkeit: 12 months

Details zu c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Andere Bezeichnung: C-Fos

Hintergrund: The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.Synonyms: Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos

Gen-ID: 2353

UniProt: P01100

Pathways: S100 Proteine