ERBB3 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN126794

Der Maus Monoklonal anti-ERBB3 Antikörper wird verwendet zum Nachweis von ERBB3 in Proben von Human. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für ERBB3 Antikörper (Cytoplasmic Domain) (ABIN126794)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERBB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 5A12

Produktdetails anti-ERBB3 Antikörper

Bindungsspezifität: AA 1250-1270, Cytoplasmic Domain

Spezifität: This antibody recognizes erbB3 phosphorylation status independent at 185 kDa in Western blot.

Produktmerkmale: Synonyms: ERBB-3, c-erbB-3, HER-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cellsurface receptor HER3, Proto-oncogene-like protein c-ErbB-3

Aufreinigung: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Peptide conjugated to hemocyanin (aa 1250-1270).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20. Positive Control: Cell lysate from untreaed SW620 cells. (See Protocols)Immunohistochemistry on Paraffin Sections. Immunocytochemistry: (1-10 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control: Cell lysate from untreated SW620 cellsFormulation: Lyophilized cell lysate from serum starved SW 620 cellsReconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Andere Bezeichnung: ERBB3 / HER3

Hintergrund: ErbB3 is a member of the EGFR/erbB receptor tyrosine kinase family. ErbB3 lacks intrinsic kinase activity due to an amino acid substitution within the ATP binding site. Upon heterodimerization with the other erbB family members, erbB3 becomes phosphorylated at multiple tyrosine residues that represent docking sites for downstream signaling proteins, e.g. PI3K, grb7 and shc. The erbB3 cytoplasmic domain contains 6 docking sites for PI3K. Thus, erbB3 is a potent activator of the PI3K/PKB signaling pathway.Synonyms: ERBB-3, HER-3, Proto-oncogene-like protein c-ErbB-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cell surface receptor HER3, c-erbB-3

Molekulargewicht: 185 kDa

Gen-ID: 2065

UniProt: P21860

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung