Lamin A/C Antikörper

Produktnummer ABIN126715

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Lamin A/C in WB, IF und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh), Xenopus laevis und Fisch.

Kurzübersicht für Lamin A/C Antikörper (ABIN126715)

Target: Lamin A/C (LMNA)

Reaktivität: Human, Rind (Kuh), Xenopus laevis, Fisch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Lamin A/C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: X67

Produktdetails anti-Lamin A/C Antikörper

Spezifität: The monoclonal antibody cocktail decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Polypeptides reacting: Lamin isotypes of Mr 60 - 75 kDa.

Aufreinigung: Protein A affinity chromatography

Immunogen: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting. Immunofluorescence microscopy. Immunohistochemistry: dilute further 1: 10 (incubation time 1 h at room temperature).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1.0 mL distilled water.

Buffer: 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: Lamin-A/C (LMNA)

Hintergrund: Nuclear Lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type Lamins and B-type Lamins. The A-type Lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and Lamin Adel 10, while the B-type Lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. Recent evidence has revealed that mutations in A-type Lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type Lamins coincides with cell differentiation and as A-type Lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type Lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32

Gen-ID: 4000

NCBI Accession: NP_005563

UniProt: P02545

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus