Cytokeratin 7 Antikörper

Produktnummer ABIN125985

Dieses Anti-Cytokeratin 7-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cytokeratin 7 in WB, IF, IHC (p) und IHC (fro). Geeignet für Human, Rind (Kuh), Schwein und Schaf.

Kurzübersicht für Cytokeratin 7 Antikörper (ABIN125985)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reaktivität: Human, Rind (Kuh), Schwein, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: Ks 7-18

Produktdetails anti-Cytokeratin 7 Antikörper

Spezifität: This antibody represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative. Detects specific subtypes of adenocarcinomas: Adenocarcimona of pancreas, gallbladder, lung, cervix, cholangio carcinoma of liver, ductal and lobular carcinoma of breast, carcinomas of ovary, transitional cell carcinoma of bladder, mesothelioma, negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed. Reacts with Mr 54,000 polypeptide (keratin K7, formerly also designated Cytokeratin 7) of human glandular epithelia.

Aufreinigung: Affinity Chromatography on Protein A .

Immunogen: Cytoskeletal proteins from cultured HeLa cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections: 1/50 (Overnight at 2-8 °C). Protease pretreatment is recommended prior to antibody application. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT). Cytological Material. Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL distilled water

Buffer: Final Solution contains PBS buffer, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Andere Bezeichnung: Cytokeratin 7

Hintergrund: Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin

Gen-ID: 3855

NCBI Accession: NP_005547

UniProt: P08729