CD59 Antikörper (CD59) (APC) Primary Antibody
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- Target Alle CD59 Antikörper anzeigen
- CD59
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD59 Antikörper ist konjugiert mit APC
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Applikation
- Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Spezifität
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The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells, it is widely present on cells in all tissues. This antibody does not compete with MEM-43/5.
HLDA IV, WS Code NL 705
HLDA V, WS Code AS S013
HLDA V, WS Code BP BP345
HLDA V, WS Code T T-103 - Kreuzreaktivität (Details)
- Human
- Immunogen
- Thymocytes and T lymphocytes
- Klon
- MEM-43
- Isotyp
- IgG2a
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- Applikationshinweise
- The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
- Kommentare
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The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- No reconstitution is necessary.
- Buffer
- The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Do not freeze.
Avoid prolonged exposure to light. - Lagerung
- 4 °C
- Informationen zur Lagerung
- Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
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Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2915-23, (2006) (PubMed).
: "GPI-anchored cell-surface molecules complexed to protein tyrosine kinases." in: Science (New York, N.Y.), Vol. 254, Issue 5034, pp. 1016-9, (1991) (PubMed).
: "Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11." in: Immunogenetics, Vol. 30, Issue 3, pp. 188-93, (1989) (PubMed).
: "
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Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2915-23, (2006) (PubMed).
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- Target
- CD59
- Andere Bezeichnung
- CD59 (CD59 Produkte)
- Synonyme
- 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20, Cd59a, Cd59b, MACIP, CD59 molecule (CD59 blood group), CD59 molecule, CD59, Cd59
- Hintergrund
- CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
- Pathways
- Komplementsystem