PUMA Antikörper (N-Term)

Produktnummer ABIN121685

Der Kaninchen Polyklonal Anti-PUMA-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, PUMA in Proben von Human und Maus zu detektieren.

Kurzübersicht für PUMA Antikörper (N-Term) (ABIN121685)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PUMA Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to the N-terminus of human PUMA alpha. AA Sequence: ARARQEGSSPEPVEG

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 2 μg/mL - 4 μg/mL, this antibody detects a band of approximately 23 kDa in K562 cell lysates. Immunohistochemistry on paraffin sections: 10 μg/mL, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: PUMA

Hintergrund: PUMA is a proapoptotic member of the BCL-2 family. PUMA is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis

UniProt: Q9BXH1

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity