beta-2 Microglobulin Antikörper

Produktnummer ABIN120759

Dieses Anti-beta-2 Microglobulin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von beta-2 Microglobulin in WB, FACS, IHC (p) und EIA. Geeignet für Human und Schwein.

Kurzübersicht für beta-2 Microglobulin Antikörper (ABIN120759)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta-2 Microglobulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: B2M-02

Produktdetails anti-beta-2 Microglobulin Antikörper

Spezifität: The antibody B2M-02 reacts with beta2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin.

Aufreinigung: Protein A chromatography

Reinheit: > 95 % (by SDS-PAGE).

Immunogen: Purified isolated human beta2-microglobulin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry (1 μg/mL): use PBL cell line as positive control. Negative control: DAUDI human lymphoma cell line, erythrocytes. Western blot (2 - 4 μg/mL): use U937 human lymphoma cell line as positive control. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both reducing and non-reducing conditions. Reducing and non-reducing conditions. ELISA. Immunohistochemistry (Paraffin): Recommended dilution: 5 m g/mL, Positive tissue: liver
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Andere Bezeichnung: beta-2-Microglobulin

Hintergrund: B2M is a 12 kDa Ig like glycoprotein. B2M non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin

Gen-ID: 567

UniProt: P61769

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process