MHC Class I RT1.Aa alpha-Chain (RT1.AA) Antikörper

Produktnummer ABIN120723

Zitiert in 1 Publikation. Der Maus Monoklonal anti-MHC Class I RT1.Aa alpha-Chain Antikörper (Klon OX-18) (ABIN120723) detektiert spezifisch MHC Class I RT1.Aa alpha-Chain in FACS, IHC (fro) und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für MHC Class I RT1.Aa alpha-Chain (RT1.AA) Antikörper (ABIN120723)

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Klon: OX-18

Produktdetails

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Rat spleen glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Referenzen

Sakurada, Osawa, Araki: "[Aortic root replacement and pulmonary vein isolation, report of a case]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 153-6, (2008) (PubMed).

Antigendetails

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Andere Bezeichnung: MHC Class I (RT1Aa)

Hintergrund: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain