MHC Class I RT1.Aa alpha-Chain (RT1.AA) Antikörper

Produktnummer ABIN120722

Es gibt 1 Publikation für dieses Produkt. Der Maus Monoklonal anti-MHC Class I RT1.Aa alpha-Chain Antikörper wird verwendet zum Nachweis von MHC Class I RT1.Aa alpha-Chain in Proben von Ratte. Er wurde validiert für FACS, IHC (fro) und IP.

Kurzübersicht für MHC Class I RT1.Aa alpha-Chain (RT1.AA) Antikörper (ABIN120722)

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Klon: OX-18

Produktdetails

Aufreinigung: Protein G affinity chromatography

Immunogen: Rat spleen glycoproteins

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on rat splenocytes). Immunohistochemistry on frozen sections: Acetone fixation is recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable for immunohistochemistry on paraffin sections. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: MRC OX-18 has been used in immunoaffinity purification of rat MHC class I molecules.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Referenzen

Sakurada, Osawa, Araki: "[Aortic root replacement and pulmonary vein isolation, report of a case]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 153-6, (2008) (PubMed).

Antigendetails

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Andere Bezeichnung: MHC Class I (RT1Aa)

Hintergrund: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain