MHC Class I RT1.Aa alpha-Chain (RT1.AA) Antikörper

Produktnummer ABIN120721

Dieser Maus Monoklonal Antikörper detektiert spezifisch MHC Class I RT1.Aa alpha-Chain in FACS, IHC (fro) und IP. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für MHC Class I RT1.Aa alpha-Chain (RT1.AA) Antikörper (ABIN120721)

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Klon: OX-18

Produktdetails

Aufreinigung: Protein G affinity chromatography

Immunogen: Rat spleen cell glycoprotein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1:50-1:100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable on paraffin embedded sections. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for up to one month or at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Antigendetails

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Andere Bezeichnung: MHC Class I (RT1Aa)

Hintergrund: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain