PAG1 Antikörper (C-Term)

Produktnummer ABIN120704

Der Maus Monoklonal anti-PAG1 Antikörper wird verwendet zum Nachweis von PAG1 in Proben von Human, Maus, Ratte und Rind (Kuh). Er wurde validiert für IP und IHC (p).

Kurzübersicht für PAG1 Antikörper (C-Term) (ABIN120704)

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAG1 Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: PAG-C1

Produktdetails anti-PAG1 Antikörper

Bindungsspezifität: C-Term

Spezifität: The antibody recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Aufreinigung: Protein-A affinity chromatography

Reinheit: > 95 % pure by SDS-PAGE

Immunogen: C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Positive control: RAJI human Burkitt lymphoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu PAG1

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Andere Bezeichnung: PAG1 / Csk-Binding Protein

Hintergrund: PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.Synonyms: CBP, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp

Gen-ID: 55824

UniProt: Q9NWQ8

Pathways: p53 Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, CXCR4-mediated Signaling Events