CD38 Antikörper

Produktnummer ABIN120671

Der Maus Monoklonal Anti-CD38 Antikörper wurde für FACS, WB, IHC (fro) und IHC (p) validiert. Er ist geeignet, CD38 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CD38 Antikörper (ABIN120671)

Target: CD38

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD38 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: HIT2

Produktdetails anti-CD38 Antikörper

Spezifität: The antibody reacts with CD38 (T10), a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes, it is an antigenic marker of lymphoid cells.

Aufreinigung: Protein-G Affinity Chromatography.

Immunogen: Peripheral blood mononuclear cells of a patient suffering with CLL

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Western Blotting (non reducing conditions): 2 mg/mL. Positive control: RAJI human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Boil for 5 min. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-CD38 Antikörper (ABIN120671)

Qu, Shen, Shen, Hu, Yang, Wang, Yan, Zhang: "Open-tubular gas chromatography using capillary coated with octadecylamine-capped gold nanoparticles." in: Analytica chimica acta, Vol. 609, Issue 1, pp. 76-81, (2008) (PubMed).

Details zu CD38

Target: CD38

Hintergrund: CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.Synonyms: ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, cADPr hydrolase 1

Gen-ID: 952

UniProt: P28907