SIT1 Antikörper

Produktnummer ABIN120658

Dieser Maus Monoklonal Antikörper detektiert spezifisch SIT1 in WB, IP und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für SIT1 Antikörper (ABIN120658)

Target: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SIT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Klon: SIT-01

Produktdetails anti-SIT1 Antikörper

Spezifität: The antibody reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.

Produktmerkmale: Synonyms: Signaling threshold-regulating transmembrane adapter 1, SHP2-interacting transmembraneadapter protein, Suppression-inducing transmembrane adapter 1

Aufreinigung: Protein-A affinity chromatography

Reinheit: > 95 % pure (by SDS-PAGE)

Immunogen: Bacterially produced recombinant intracellular fragment of human SIT.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (intracellular staining). Immunoprecipitation. Western Blotting: Western blot analysis demonstrated that SIT is expressed as anapproximately 40 kDa protein that is reduced to approximately 20 kDa by endoglycosidasetreatment.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu SIT1

Target: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Andere Bezeichnung: SIT1 / SIT

Hintergrund: SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.Synonyms: SHP2-interacting transmembrane adapter protein, Signaling threshold-regulating transmembrane adapter 1, Suppression-inducing transmembrane adapter 1

Gen-ID: 27240

UniProt: Q9Y3P8