PAG1 Antikörper (AA 97-432)

Produktnummer ABIN120652

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PAG1 in WB, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PAG1 Antikörper (AA 97-432) (ABIN120652)

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MEM-255

Produktdetails anti-PAG1 Antikörper

Bindungsspezifität: AA 97-432

Spezifität: The antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).

Aufreinigung: Protein-A affinity chromatography

Reinheit: > 95 % pure (by SDS-PAGE)

Immunogen: Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 2 mg/mL (intracellular staining, permeabilization required). Western Blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS- PAGE gels anomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu PAG1

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Andere Bezeichnung: PAG1 / Csk-Binding Protein

Hintergrund: PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.Synonyms: CBP, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp

Gen-ID: 55824

UniProt: Q9NWQ8

Pathways: p53 Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, CXCR4-mediated Signaling Events