CD47 Antikörper

Produktnummer ABIN120599

Der Maus Monoklonal Anti-CD47-Antikörper wurde für FACS, WB und IHC (fro) validiert. Er ist geeignet, CD47 in Proben von Human zu detektieren.

Kurzübersicht für CD47 Antikörper (ABIN120599)

Target: CD47

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD47 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: MEM-122

Produktdetails anti-CD47 Antikörper

Spezifität: The antibody reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (Thrombospondin Receptor, immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.

Aufreinigung: CHT-Chromatography and Precipitation Methods

Immunogen: COS-7 (African green monkey) cells

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Flow cytometry. Western blotting (non-reducing conditions). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C. Do not freeze.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD47

Target: CD47

Andere Bezeichnung: CD47

Hintergrund: CD47 is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein a, SIRPa), an immune inhibitory receptor on macrophages, this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.Synonyms: Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, OA3

Gen-ID: 961

UniProt: Q08722