Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
Spezifität
This antibody recognises the beta chain of the rat CD8 cell surface antigen. This antibody is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.
Aufreinigung
Affinity chromatography on Protein G
Immunogen
Rat/mouse T cell hybrids expressing CD8. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Flow Cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD8 is expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain