CD3D Antikörper

Produktnummer ABIN120371

Der Maus Monoklonal Anti-CD3D-Antikörper wurde für IP, FACS, IHC (p), Func und IHC (fro) validiert. Er ist geeignet, CD3D in Proben von Ratte zu detektieren.

Kurzübersicht für CD3D Antikörper (ABIN120371)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3D Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 1F4

Produktdetails anti-CD3D Antikörper

Spezifität: SM253PS recognizes Rat CD3, a 25 kDa antigen which is found on rat T-cells. SM253PS does not react with Rat B cells. In immunohistology this antibody stains Rat thymus tissues strongly in the medulla and weakly in the cortex.

Aufreinigung: Ammonium Sulphate Precipitation

Immunogen: F344 rat T cells stimulated with PMA (TPA) and calcium ionophore. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Flow cytometry (Use 10 μL of 1/10 - 1/25 diluted antibody to label 10^6 cells in 100 μL). Immunohistochemistry on frozen sections (1/10 - 1/25) and paraffin sections (1/10 - see reference 3). In immunohistology this antibody stains rat thymus tissues strongly in the medulla and weakly in the cortex. Immunoprecipitation. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T- cells in the presence of PMA (1). We recommend the use of ABIN120367 for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Andere Bezeichnung: CD3 delta Chain

Hintergrund: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: CD3D, T-cell surface glycoprotein CD3 delta chain, T3D

Gen-ID: 25710

NCBI Accession: NP_037301

UniProt: P19377

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events