MADCAM1 Antikörper

Produktnummer ABIN120237

Dieses Anti-MADCAM1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MADCAM1 in WB, FACS, IHC (p), Func und EIA. Geeignet für Human.

Kurzübersicht für MADCAM1 Antikörper (ABIN120237)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MADCAM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Klon: 314G8

Produktdetails anti-MADCAM1 Antikörper

Spezifität: This antibody recognises Human mucosal addressin cell adhesion molecule 1 (MAdCAM-1). Clone 314G8 does not recognise MAdCAM-1 in the gut lamina propria. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.

Aufreinigung: Affinity Chromatography on Protein G

Endotoxin-Niveau: Low Endotoxin

Immunogen: Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: this antibody detects a band of approximately 60 kDa. Functional Assays. Flow Cytometry: 1/10 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining, This product does not require antigen retrieval prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 without preservatives or stabilizers

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Andere Bezeichnung: MAdCAM1

Hintergrund: MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1

Gen-ID: 8174

NCBI Accession: NP_570116

UniProt: Q13477