TUBA1B Antikörper

Produktnummer ABIN120144

Der Ratte Monoklonal anti-TUBA1B Antikörper (Klon YL1-2) (ABIN120144) detektiert spezifisch TUBA1B in WB, IF, IHC (fro), IP, EIA und RIA. Dieser Antikörper reagiert spezifisch mit Proben aus Avian, Säugetier und Saccharomyces cerevisiae.

Kurzübersicht für TUBA1B Antikörper (ABIN120144)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reaktivität: Avian, Säugetier, Saccharomyces cerevisiae

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TUBA1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Klon: YL1-2

Produktdetails anti-TUBA1B Antikörper

Spezifität: SM2202P recognises the alpha subunit of Tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized Actin (5).

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Yeast Tubulin. Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections. ELISA (1/100-1/1000, routinely tested in ELISA on Tubulin) (3). Immunoprecipitation. Radioimmunoassay. Clone YL1/2 has also been reported to work in western blotting (3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Andere Bezeichnung: alpha Tubulin / TUBA1B

Hintergrund: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

Gen-ID: 10376

NCBI Accession: NP_006073

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase