ITGB1 Antikörper

Produktnummer ABIN120030

Dieses Anti-ITGB1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ITGB1 in WB, FACS, IP, IHC (p), IHC (fro) und EIA. Geeignet für Human und Affe. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für ITGB1 Antikörper (ABIN120030)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: JB1B

Produktdetails anti-ITGB1 Antikörper

Aufreinigung: Ammonium Sulphate Precipitation

Immunogen: Purified Human beta-1 integrin. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1 μg/10^6cells. Immunoprecipitation: 1 - 5 μg/mL. Immunohistochemistry on frozen sections: 5 μg/mL. ELISA: 0.1-1 μg/mL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-ITGB1 Antikörper (ABIN120030)

Haglund, Tillgren, Addis, Wenglén, Recklies, Heinegård: "Identification and characterization of the integrin alpha2beta1 binding motif in chondroadherin mediating cell attachment." in: The Journal of biological chemistry, Vol. 286, Issue 5, pp. 3925-34, (2011) (PubMed).

Details zu ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Andere Bezeichnung: CD29 / Integrin beta-1

Hintergrund: Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.Synonyms: FNRB, Fibronectin receptor subunit beta, ITGB1, Integrin VLA-4 subunit beta, MDF2, MSK12

Gen-ID: 9606

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interaktom