This antibody recognizes Mouse Aminopeptidase N. Clone R3-63 has been reported to block mouse APN enzyme activity (1). We recommend the use of an Azide Free antibody (SM1856A) for this purpose.
Aufreinigung
Protein G Chromatography
Immunogen
Mouse intestinal APN Remarks: Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line.
Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Murine Aminopeptidase N (APN) is a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.Synonyms: ANPEP, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150