alpha Defensin 1 Antikörper

Produktnummer ABIN118975

Produktdetails anti-alpha Defensin 1 Antikörper

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Defensin 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Several Defensins are recognized. This antibody recognises a family of Cyclic Peptides in Neutrophils. Human: Defensin 1-3 (HNP-1 to HNP-3) in Human Neutrophils. Synthetic Defensin-1 and -2 (Bachem, Bubendorf CH) stain also positively. Not tested with Defensin-4. A side reaction to elastin has been observed in humans. Other: not tested. Defensins have been described in rabbits (NP-1 to NP-5, NP3a = Corticostatin), and in mice (cryptodin).

Aufreinigung: Affinity chromatography

Immunogen: Native defensins. The epitope has not been further characterized.

Klon: DEF-3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 0.2 μg/mL (1/1000). Immunohistochemistry on Paraffin Sections: 0.5 μg/mL (1/400), Proteinase K pretreatmentfor antigen retrieval is recommended. Positive Control: Human tonsil. Has been discribed to work in FACS with permeabilized cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 5 mg/mL BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilised antibody at 2-8 °C and after reconstitution at -20 °C.

Details zu alpha Defensin 1

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Andere Bezeichnung: Defensin alpha 1 (DEFA1 Produkte)

Synonyme: DEF1 antikoerper, DEFA2 antikoerper, HNP-1 antikoerper, HP-1 antikoerper, HP1 antikoerper, MRS antikoerper, Defcr antikoerper, Defcr1 antikoerper, DEFA1 antikoerper, Defa antikoerper, Def antikoerper, GB19392 antikoerper, defensin alpha 1 antikoerper, defensin, alpha 1 antikoerper, defensin alpha 5 antikoerper, alpha-defensin 1A antikoerper, defensin 1 antikoerper, neutrophil defensin 1 antikoerper, DEFA1 antikoerper, Defa1 antikoerper, Defa5 antikoerper, MNP1A antikoerper, Def1 antikoerper, LOC107973256 antikoerper

Hintergrund: Defensins are a family of cyclic peptides containing 29-35 amino acids (MW < 3500) which tend to form aggregates. Four of these peptides are described in humans (HNP-1 to 4), six in rabbits (NP-1 to 5). Synonyms are MCP-1 for NP-1, MCP-2 for NP-2 and corticostatin for NP-3. Biological Functions: Various functions have been described for defensins. They are antibacterial, antifungal, chemotactic for monocytes, inhibitory for ACTH-induced corticosteroid synthesis and cytotoxic for cells. Defensins are inhibited by glucosaminoglycans (self protection for cells) and high concentrations of Ca2+ ions.Synonyms: DEF-1, DEF-3, DEF-A1, DEF-A2, DEF-A3, DEF1, DEF3, DEFA-1, DEFA-2, DEFA-3, DEFA1, DEFA2, DEFA3, Defensin alpha 1, Defensin alpha 3, HNP-1, HNP-3, HNP1, HNP3, HP-1, HP-3, HP1, HP3, MRS, Neutrophil defensin 1, Neutrophil defensin 3

Gen-ID: 1667

NCBI Accession: NP_001035965

UniProt: P59665

Pathways: Cellular Response to Molecule of Bacterial Origin