CD56 Antikörper (Biotin)

Produktnummer ABIN118741

Produktdetails anti-CD56 Antikörper (Biotin)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD56 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Spezifität: Recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM) (1).

Immunogen: Human retinoblastoma tumor tissue, membrane fraction

Klon: ERIC-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Studies of neural cell adhesion molecule. May also be used to study isoforms of NCAM expressed on different tumor types. Western blot for analysis of isoforms expressed, did not react with any of the leukemias or lymphomas tested (1). Flow cytometric applications (1 μg to stain 6 x 10e5 cells). Not for use in IHC with formalin-fixed, paraffin embedded sections. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20 °C) for 5 minutes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 µg/mL (OD280nm)

Buffer: 0.01 M PBS, pH 7.2, containing 2.0 mM EDTA, 1 % BSA as stabilizer and 0.09 % Sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Andere Bezeichnung: CD56 (NCAM1 Produkte)

Hintergrund: NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.Synonyms: N-CAM-1, NCAM, NCAM-1, Neural cell adhesion molecule 1

Gen-ID: 4684

NCBI Accession: NP_000606

UniProt: P13591