CD53 Antikörper

Produktnummer ABIN118730

Der Maus Monoklonal Anti-CD53-Antikörper wurde für FACS, WB, IP, Func und IHC (fro) validiert. Er ist geeignet, CD53 in Proben von Human zu detektieren.

Kurzübersicht für CD53 Antikörper (ABIN118730)

Target: CD53

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD53 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: MEM-53

Produktdetails anti-CD53 Antikörper

Spezifität: The antibody reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes, it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).

Aufreinigung: Protein-A affinity chromatography

Reinheit: > 95 % pure (by SDS-PAGE)

Immunogen: Leukocytes of pacient suffering from a LGL-type leukemia

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Immunoprecipitation. Western Blotting. Immunohistochemistry on frozen sections. Suitable for discrimination of lymphomas from other tumors. Functional Application. The antibody induces activation of monocytes and B lymphocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD53

Target: CD53

Andere Bezeichnung: CD53

Hintergrund: CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.Synonyms: Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, TSPAN25, Tetraspanin-25

Gen-ID: 963

NCBI Accession: NP_000551

UniProt: P19397