CD33 Antikörper

Produktnummer ABIN118637

Der Maus Monoklonal Anti-CD33-Antikörper wurde für FACS, WB, IP und IHC (fro) validiert. Er ist geeignet, CD33 in Proben von Human zu detektieren.

Kurzübersicht für CD33 Antikörper (ABIN118637)

Target: CD33

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD33 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: WM53

Produktdetails anti-CD33 Antikörper

Spezifität: Recognizes the CD33 cell surface glycoprotein, a 67 kDa molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Human AML cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections (1/50-1/100): Overnight incubation recommended. Positive control tissue: lymphatic tissue. Not suitable on Paraffin Embedded Sections. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD33

Target: CD33

Andere Bezeichnung: CD33 / SIGLEC3

Hintergrund: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

Gen-ID: 945

NCBI Accession: NP_001763

UniProt: P20138