CD8B Antikörper

Produktnummer ABIN118499

Der Maus Monoklonal Anti-CD8B-Antikörper wurde für FACS, WB und IP validiert. Er ist geeignet, CD8B in Proben von Human und Affe zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CD8B Antikörper (ABIN118499)

Target: CD8B (CD8b Molecule (CD8B))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD8B Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Klon: 2ST8-5H7

Produktdetails anti-CD8B Antikörper

Spezifität: This antibody recognises the CD8 cell surface antigen. The antibody binds to the beta chain, recognising an epitope that is dependent upon expression of both CD8 alpha and CD8 beta. Reacts with Cynomolgus Monkey.

Aufreinigung: Purified

Immunogen: Normal Human T lymphocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CD8B Antikörper (ABIN118499)

Heigele, Schindler, Gnanadurai, Leonard, Collins, Kirchhoff: "Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins." in: Journal of virology, Vol. 86, Issue 1, pp. 36-48, (2012) (PubMed).

Details zu CD8B

Target: CD8B (CD8b Molecule (CD8B))

Andere Bezeichnung: CD8 beta

Hintergrund: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain

Gen-ID: 926

NCBI Accession: NP_001171571

UniProt: P10966

Pathways: T-Zell Rezeptor Signalweg