Albumin Antikörper (HRP)

Produktnummer ABIN118089

Der HRP-konjugierte Kaninchen Polyklonal anti-Albumin Antikörper (ABIN118089) detektiert spezifisch Albumin in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Albumin Antikörper (HRP) (ABIN118089)

Target: Albumin (ALB)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Albumin Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Albumin Antikörper (HRP)

Spezifität: This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Serum Albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified human serum albumin IgG, and human Serum.

Aufreinigung: Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated below.

Immunogen: Human Serum Albumin.

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (western or dot blot), ELISA, Immunoperoxidase ElectronMicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 μg of Human Albumin IgG in a standardcapture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/10,000 of the reconstitution concentration is suggested

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL of deionized water (or equivalent).

Konzentration: 1.0 mg/mL (before lyophilization by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Gentamicin Sulfate as preservative and Polyethylen Glycol (PEG-8000) as stabilizer.

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin

Hintergrund: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

Gen-ID: 213

NCBI Accession: NP_000468

UniProt: P02768

Pathways: Lipid Metabolism