ING1 Antikörper (AA 285-296)

Produktnummer ABIN118055

Produktdetails anti-ING1 Antikörper

Target: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Bindungsspezifität: AA 285-296

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ING1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p33 ING1 protein and is 100 % homologous to all four isoforms (A, B, C, and D) on record for this protein. Cross reactivity with p33 ING1 protein from mouse and rat sources is expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p33 ING1 proteins from other sources is not known.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 285-296 of Human p33 ING1 protein (Inhibitor of growth family, member 1). Remarks: This sequence shows 100% homology to all splice variants for ING1.

Anwendungsinformationen

Applikationshinweise: This affinity-purified antibody has been tested for use in ELISA (1: 4,000-1: 14,000) andWestern blotting (1: 500-1: 3,000) using over expressed cell lysates. Expect a bandapproximately 47 kDa, 33 kDa or 24 kDa in size corresponding to variants of ING1 bywestern blotting in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (UVabs. 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Details zu ING1

Target: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Andere Bezeichnung: ING1 (ING1 Produkte)

Synonyme: p24ING1c antikoerper, p33 antikoerper, p33ING1 antikoerper, p33ING1b antikoerper, p47 antikoerper, p47ING1a antikoerper, p33ING1c antikoerper, zgc:136918 antikoerper, ING1 antikoerper, 2610028J21Rik antikoerper, AA407184 antikoerper, AI875420 antikoerper, mING1h antikoerper, p33Ing1 antikoerper, p37Ing1b antikoerper, xing1 antikoerper, inhibitor of growth family member 1 antikoerper, inhibitor of growth family, member 1 antikoerper, inhibitor of growth family member 1 S homeolog antikoerper, ING1 antikoerper, Ing1 antikoerper, ing1 antikoerper, ing1.S antikoerper

Hintergrund: P33 ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53-signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. The accession number listed below is for variant (4) that encodes the longest isoform (D). Other synonyms for p33 ING1 include p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, growth inhibitor ING1, inhibitor of growth 1, tumor suppressor ING1 and growth inhibitory protein ING1.Synonyms: ING-1, Inhibitor of growth protein 1

Gen-ID: 3621

NCBI Accession: NP_005528

UniProt: Q9UK53

Pathways: Protein targeting to Nucleus, Autophagie