Albumin Antikörper

Produktnummer ABIN118031

Der Kaninchen Polyklonal Anti-Albumin Antikörper wurde für EIA validiert. Er ist geeignet, Albumin in Proben von Human zu detektieren.

Kurzübersicht für Albumin Antikörper (ABIN118031)

Target: Albumin (ALB)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Albumin Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA)

Produktdetails anti-Albumin Antikörper

Spezifität: Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was dialyzed against PBS with sodium azide, concentrated by Amicon ultrafiltration, 0.2 μm filtered, aseptically filled, and stored at 2-8° C. An aliquot was submitted for final qualification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Human Serum Albumin.

Anwendungsinformationen

Applikationshinweise: Suitable for most Immunological methods. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.04 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative without stabilizers.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin

Hintergrund: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

Gen-ID: 213

NCBI Accession: NP_000468

UniProt: P02768

Pathways: Lipid Metabolism