DNMT3L Antikörper (AA 152-164)

Produktnummer ABIN118012

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DNMT3L in WB, IP und EIA. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für DNMT3L Antikörper (AA 152-164) (ABIN118012)

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DNMT3L Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Produktdetails anti-DNMT3L Antikörper

Bindungsspezifität: AA 152-164

Sequenz: Peptide: G-L-L-Q-R-R-R-K-W-R-S-Q-L Protein: 1 maaipaldpe aepsmdvilv gsselsssvs pgtgrdliay evkanqrnie diciccgslq 61 vhtqhplfeg gicapckdkf ldalflyddd gyqsycsicc sgetllicgn pdctrcycfe 121 cvdslvgpgt sgkvhamsnw vcylclpssr sgllqrrrkw rsqlkafydr esenplemfe 181 tvpvwrrqpv rvlslfedik keltslgfle sgsdpgqlkh vvdvtdtvrk dveewgpfdl 241 vygatpplgh tcdrppswyl fqfhrllqya rpkpgspgpf fwmfvdnlvl nkedldvasr 301 flemepvtip dvhggslqna vrvwsnipai rsrhwalvse eelsllaqnk qssklaakwp 361 tklvkncflp lreyfkyfst eltssl

Spezifität: This antibody reacts with DNMT3L.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse (76 %).
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase

Immunogen: A synthetic peptide corresponding to aa 152-164 of Human DNMT3L.

Anwendungsinformationen

Applikationshinweise: This product has been assayed by ELISA, Western blotting and Immunoprecipitation. The antibody was tested by ELISA against 0.1 μg of the immunizing peptide. A1/1,000-1/3,000 dilution of the antibody is recommended for this assay. For Western blotting, first Immunoprecipitate (1/100 dilution) the antigen using this

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.43 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Shelf live: One year from despatch.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use.

Details zu DNMT3L

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Andere Bezeichnung: DNMT3L

Hintergrund: DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.Synonyms: DNA (cytosine-5)-methyltransferase 3-like

Gen-ID: 29947

NCBI Accession: NP_037501

UniProt: Q9UJW3