RPA2 Antikörper (AA 115-129)

Produktnummer ABIN118008

Dieses Anti-RPA2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RPA2 in WB und EIA. Geeignet für Saccharomyces cerevisiae.

Kurzübersicht für RPA2 Antikörper (AA 115-129) (ABIN118008)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-RPA2 Antikörper

Bindungsspezifität: AA 115-129

Sequenz: Peptide sequence: S-S-G-K-G-Y-G-S-Q-V-A-Q-Q-F-E

Spezifität: This affinity purified antibody is directed against yeast RFA2. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was purified against the phosphorylated form of the immunizing peptide and then cross adsorbed against the non-phosphorylated form of the peptide. This antibody consists of antibodies eluted from the non-phospho peptide column.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively. Yeast RFA2 is phosphorylated at the G1/S transition and is dephosphorylated at mitosis.

Anwendungsinformationen

Applikationshinweise: This affinity-purified antibody was tested for use in western blotting against RFA2containing cell lysates and by ELISA against the immunizing peptide. Reactivity in otherimmunoassays is unknown. This product was assayed by western blotting against RFA2containing cell lysates. A 1: 100 to 1: 500 dilution is recommended for this assay. Thisproduct was also assayed by ELISA against 0.1 μg of the phosphorylated andnonphosphorylated form of the immunizing peptide. A 1: 1,000 to 1: 3,000 dilution of theantibody is recommended against both forms of the peptide for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.12 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C.

Details zu RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Andere Bezeichnung: RPA2

Hintergrund: Synonyms: REPA2, RF-A protein 2, RFA2, RP-A p32, RP-A p34, RPA32, RPA34, Replication factor A protein 2, Replication protein A, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit

Gen-ID: 6118

NCBI Accession: NP_002937

UniProt: P15927

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA