NEK2 Antikörper (AA 287-299)

Produktnummer ABIN117981

Der Kaninchen Polyklonal anti-NEK2 Antikörper (ABIN117981) detektiert spezifisch NEK2 in EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für NEK2 Antikörper (AA 287-299) (ABIN117981)

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEK2 Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA)

Produktdetails anti-NEK2 Antikörper

Bindungsspezifität: AA 287-299

Sequenz: G-R-Q-L-G-E-P-E-K-S-Q-D-S Remarks: Human NEK2 is a protein kinase involved in mitotic regulation at the G2-M transition and/or at meiosis.

Spezifität: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 287-299 of Human NEK2 protein.

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA against the immunizingpeptide. Reactivity in other immunoassays is unknown. This product has been assayed byELISA against 0.1 μg of the immunizing peptide. A 1: 2,000 to 1: 8,000 dilution of theantibody is recommended for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Details zu NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Andere Bezeichnung: NEK2

Hintergrund: NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers.Synonyms: HSPK 21, NLK1, NimA-related protein kinase 2, Serine/threonine-protein kinase Nek2

Gen-ID: 4751

NCBI Accession: NP_001191111

UniProt: P51955

Pathways: M Phase