Aurora Kinase B Antikörper (AA 227-238)

Produktnummer ABIN117978

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-Aurora Kinase B Antikörper (ABIN117978) detektiert spezifisch Aurora Kinase B in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Aurora Kinase B Antikörper (AA 227-238) (ABIN117978)

Target: Aurora Kinase B (AURKB)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aurora Kinase B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Aurora Kinase B Antikörper

Bindungsspezifität: AA 227-238

Sequenz: Human Aurora B Kinase, 344 aa, predicted MW 39.3kDa 1 maqkensypw pygrqtapsg lstlpqrvlr kepvtpsalv lmsrsnvqpt aapgqkvmen 61 ssgtpdiltr hftiddfeig rplgkgkfgn vylarekksh fivalkvlfk sqiekegveh 121 qlrreieiqa hlhhpnilrl ynyfydrrri ylileyaprg elykelqksc tfdeqrtati 181 meeladalmy chgkkvihrd ikpenlllgl kgelkiadfg wsvhapslrr ktmcgtldyl 241 ppemiegrmh nekvdlwcig vlcyellvgn ppfesashne tyrrivkvdl kfpasvptga 301 qdliskllrh npserlplaq vsahpwvran srrvlppsal qsva

Spezifität: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Aurora Kinase B protein. No reaction is expected against Aurora Kinase A. However, 100 % sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 227-238 of Human Aurora Kinase B protein.

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA (1: 5,000-1: 20,000) and byWestern blot (1: 500-1: 2,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B bywestern blotting in the appropriate cell lysate or extract. Human brain tissue lysate or HeLacell lysate can be used as a positive control.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.45 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Referenzen für anti-Aurora Kinase B Antikörper (ABIN117978)

Honda, Asato, Tanaka, Endo, Nishimura, Ito: "Vidian nerve schwannoma with middle cranial fossa extension resected via a maxillary swing approach." in: Head & neck, (2008) (PubMed).

Details zu Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Andere Bezeichnung: Aurora Kinase B

Hintergrund: Aurora Kinase B (also known as Aurora- and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.Synonyms: AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12

Gen-ID: 9212

NCBI Accession: NP_004208

UniProt: Q96GD4

Pathways: T-Zell Rezeptor Signalweg, Zellzyklus, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades