DDB1 Antikörper (AA 198-213)

Produktnummer ABIN117954

Der Kaninchen Polyklonal anti-DDB1 Antikörper (ABIN117954) detektiert spezifisch DDB1 in WB, IP und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für DDB1 Antikörper (AA 198-213) (ABIN117954)

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Produktdetails anti-DDB1 Antikörper

Bindungsspezifität: AA 198-213

Sequenz: Human DDB1 (1140 aa 127.0. kDa): 1 msynyvvtaq kptavngcvt ghftsaedln lliakntrle iyvvtaeglr pvkevgmygk 61 iavmelfrpk geskdllfil takynacile ykqsgesidi itrahgnvqd rigrpsetgi 121 igiidpecrm iglrlydglf kvipldrdnk elkafnirle elhvidvkfl ygcqapticf 181 vyqdpqgrhv ktyevslrek efnkgpwkqe nveaeasmvi avpepfggai iigqesityh 241 ngdkylaiap piikqstivc hnrvdpngsr yllgdmegrl fmlllekeeq mdgtvtlkdl 301 rvellgetsi aecltyldng vvfvgsrlgd sqlvklnvds neqgsyvvam etftnlgpiv 361 dmcvvdlerq gqgqlvtcsg afkegslrii rngigiheha sidlpgikgl wplrsdpnre --- 661 ssnhklvfsn vnlkevnymc plnsdgypds lalannstlt igtideiqkl hirtvplyes 721 prkicyqevs qcfgvlssri evqdtsggtt alrpsastqa lsssvssskl fssstaphet 781 sfgeevevhn lliidqhtfe vlhahqflqn eyalslvsck lgkdpntyfi vgtamvypee 841 aepkqgrivv fqysdgklqt vaekevkgav ysmvefngkl lasinstvrl yewttekdvr 901 tecnhynnim alylktkgdf ilvgdlmrsv lllaykpmeg nfeeiardfn pnwmsaveil 961 dddnflgaen afnlfvcqkd saattdeerq hlqevglfhl gefvnvfchg slvmqnlget 1021 stptqgsvlf gtvngmiglv tslseswynl lldmqnrlnk viksvgkieh sfwrsfhter 1081 ktepatgfid gdliesfldi srpkmqevva nlqyddgsgm kreataddli kvveeltrih

Spezifität: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse DDB1. Cross reactivity with DDB1 from other sources is not known.

Aufreinigung: Delipidation and defibrination.

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH.

Anwendungsinformationen

Applikationshinweise: This antibody reacts with human and mouse DDB1 by Western blot (1: 500 - 1: 1,000) andImmunoprecipitation. The antibody immunoprecipitates in vitro translated protein andprotein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation ofrelated proteins has not been tested. A 127.0 kDa band corresponding to human DDB1 isdetected. Most cell lines expressing DDB1 can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 85 mg/mL (by Refractometry)

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C.

Details zu DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Andere Bezeichnung: DDB1 / XAP1

Hintergrund: DDB1 is also known as damage-specific DNA binding protein 1, DDB p127 subunit, DDBa, UV damaged DNA-binding protein 1, UV-DDB 1, Xeroderma pigmentosum group E complementing protein, XPCe, X-associated protein 1 and XAP-1. The DDB1 gene encodes the large subunit (p127) of DNA damage-binding protein, which is a heterodimer, composed of a large and a small subunit (p48 DDB2). This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients.Synonyms: DDBa, DNA damage-binding protein 1, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-DDB 1, UV-damaged DNA-binding factor, XAP-1, XPCe, XPE-BF, Xeroderma pigmentosum group E-complementing protein

Gen-ID: 100290337

NCBI Accession: NP_001914

UniProt: Q16531

Pathways: DNA Reparatur