ANAPC11 Antikörper (C-Term)

Produktnummer ABIN117941

Der Kaninchen Polyklonal Anti-ANAPC11-Antikörper wurde für WB, EIA und IP validiert. Er ist geeignet, ANAPC11 in Proben von Human zu detektieren.

Kurzübersicht für ANAPC11 Antikörper (C-Term) (ABIN117941)

Target: ANAPC11 (Anaphase Promoting Complex Subunit 11 (ANAPC11))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ANAPC11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Produktdetails anti-ANAPC11 Antikörper

Bindungsspezifität: AA 76-84, C-Term

Sequenz: Human APC11 (84 aa 9.8 kDa): 1 mkvkikcwng vatwlwvand encgicrmaf ngccpdckvp gddcplvwgq cshcfhmhci 61 lkwlhaqqvq qhcpmcrqew kfke

Spezifität: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This antibody reacts with human and mouse APC11. Cross reactivity may also occur with APC11 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.

Aufreinigung: Delipidation and defibrination.

Immunogen: This APC11 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH.

Anwendungsinformationen

Applikationshinweise: Western blot (1: 500-1: 1,000). ELISA (1: 2,000-1: 10,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC2) does occur. A 9.8 kDa bandcorresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 canbe used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 85 mg/mL (by Refractometry)

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -80 °C for longer.

Details zu ANAPC11

Target: ANAPC11 (Anaphase Promoting Complex Subunit 11 (ANAPC11))

Andere Bezeichnung: APC11 / ANAPC11

Hintergrund: APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart, in moderate levels in brain, kidney, and liver, and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.Synonyms: Anaphase-promoting complex subunit 11, Cyclosome subunit 11, HSPC214, Hepatocellular carcinoma-associated RING finger protein

Gen-ID: 51529

NCBI Accession: NP_001002244

UniProt: Q9NYG5