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RAD52 Antikörper (AA 360-375)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RAD52 in WB, IF und EIA. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN117934

Kurzübersicht für RAD52 Antikörper (AA 360-375) (ABIN117934)

Target

Alle RAD52 Antikörper anzeigen
RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Reaktivität

  • 51
  • 11
  • 3
  • 3
  • 1
  • 1
Human

Wirt

  • 48
  • 3
Kaninchen

Klonalität

  • 46
  • 5
Polyklonal

Konjugat

  • 34
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser RAD52 Antikörper ist unkonjugiert

Applikation

  • 43
  • 31
  • 12
  • 8
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
  • Bindungsspezifität

    • 9
    • 7
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 360-375

    Sequenz

    Human RAD52, 419 aa, predicted MW 46.3 kDa: 1 msgteeailg grdshpaagg gsvlcfgqcq ytaeeyqaiq kalrqrlgpe yissrmaggg 61 qkvcyieghr vinlanemfg yngwahsitq qnvdfvdlnn gkfyvgvcaf vrvqlkdgsy 121 hedvgygvse glkskalsle karkeavtdg lkralrsfgn algncildkd ylrslnklpr 181 qlplevdltk akrqdlepsv eearynscrp nmalghpqlq qvtspsrpsh avipadqdcs 241 srslsssave seathqrklr qkqlqqqfre rmekqqvrvs tpsaekseaa ppappvthst 301 pvtvseplle kdflagvtqe liktlednse kwavtpdagd gvvkpssrad paqtsdtlal 361 nnqmvtqnrt phsvchqkpq aksgswdlqt ysadqrttgn weshrksqdm kkrkydpsy

    Spezifität

    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human RAD52 protein. Cross reactivity with RAD52 protein from non-human sources is not expected based on a lack of homology (BLAST) with other known proteins.

    Aufreinigung

    Immunoaffinity chromatography.

    Immunogen

    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 360-375 of the Human Rad 52 protein.
  • Applikationshinweise

    This affinity purified antibody has been tested for use in Western blotting (1: 500-1: 2,000)using HeLa nuclear extracts. The antibody is also functional by ELISA (1: 5,000-1: 20,000). Dilutions for Western blotting represent a starting point dilution and further optimizationmay be required. The antibody detects a band of approximately 63.0 kDa (predictedmolecular weight: 46.3 kDa). Specific band detection by Western blot is blocked bypeptide competition by pre-incubating the antibody with the immunizing peptide prior toreaction with the membrane. Reactivity in other immunoassays is unknown.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1.0 mg/mL (by UV absorbance at 280 nm)

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
  • Target

    RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

    Andere Bezeichnung

    RAD52

    Hintergrund

    The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).Synonyms: DNA repair protein RAD52 homolog

    Gen-ID

    5893

    NCBI Accession

    NP_602296

    UniProt

    P43351

    Pathways

    DNA Reparatur
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