PPARG Antikörper (N-Term)

Produktnummer ABIN117930

Der Kaninchen Polyklonal anti-PPARG Antikörper (ABIN117930) detektiert spezifisch PPARG in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für PPARG Antikörper (N-Term) (ABIN117930)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPARG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-PPARG Antikörper

Bindungsspezifität: N-Term

Sequenz: Peptide sequence: M-G-E-T-L-G-D-S-P-I-D-P-E-S-D-S

Spezifität: This antibody is directed against Human PPAR gamma isoform 2 protein. A BLAST analysis was used to suggest reactivity with this protein from Human and Macaque based on 100 % homology for the immunogen sequence. Cross reactivity with PPARg2 protein from Dog and Swine is likely due to 94 % homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from Mouse. Mouse PPARg2 shows 87 % homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

Aufreinigung: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of Human PPAR gamma 2. PPAR gamma 2 is a 55 kDa protein.

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA (1: 4,000-1: 40,000) and byWestern blot (1: 1,000-1: 3,000). Specific conditions for reactivity should be optimized bythe end user. Expect a single band approximately 55 kDa in size corresponding to PPARγ2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Details zu PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Andere Bezeichnung: PPAR-gamma

Hintergrund: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. The target genes of PPAR-alpha and PPAR-delta are primarily involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.Synonyms: NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma

Gen-ID: 5468

NCBI Accession: NP_005028

UniProt: P37231

Pathways: MAPK Signalweg, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation