Phosphotyrosine Antikörper

Produktnummer ABIN1177365

Zitiert in 3+ Publikationen. Der Maus Monoklonal anti-Phosphotyrosine Antikörper (Klon PY20) (ABIN1177365) detektiert spezifisch Phosphotyrosine in ICS.

Kurzübersicht für Phosphotyrosine Antikörper (ABIN1177365)

Target: Phosphotyrosine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Intracellular Staining (ICS)

Klon: PY20

Produktdetails anti-Phosphotyrosine Antikörper

Marke: BD Phosflow™

Aufreinigung: Purified from tissue culture supernatant or ascites by affinity chromatography

Isotyp: IgG2b

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Referenzen für anti-Phosphotyrosine Antikörper (ABIN1177365)

Arvidsson, Rupp, Nånberg, Downward, Rönnstrand, Wennström, Schlessinger, Heldin, Claesson-Welsh: "Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation." in: Molecular and cellular biology, Vol. 14, Issue 10, pp. 6715-26, (1994) (PubMed).

Fan, Mendelsohn, Masui, Kumar: "Regulation of epidermal growth factor receptor in NIH3T3/HER14 cells by antireceptor monoclonal antibodies." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21073-9, (1993) (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, (1988) (PubMed).

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.