TCR beta Antikörper

Produktnummer ABIN1177302

Dieser Anti-TCR beta Antikörper ist ein Armenischer Hamster Monoklonal Antikörper zur Detektion von TCR beta in FACS, WB, IP, IHC (fro) und IHC (f). Geeignet für Maus. Dieses Primary Antibody wurde in 13+ Publikationen zitiert.

Kurzübersicht für TCR beta Antikörper (ABIN1177302)

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser TCR beta Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: H57-597

Produktdetails anti-TCR beta Antikörper

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: TCR affinity-purified from mouse T-cell hybridoma DO-11.10

Isotyp: IgG2 lambda

Anwendungsinformationen

Applikationshinweise: Flow cytometry: It has been observed that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3e and anti-TCR beta chain mAbs to detect the T cell receptor on immature thymocytes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-TCR beta Antikörper (ABIN1177302)

Saint-Ruf, Panigada, Azogui, Debey, von Boehmer, Grassi: "Different initiation of pre-TCR and gammadeltaTCR signalling." in: Nature, Vol. 406, Issue 6795, pp. 524-7, (2000) (PubMed).

Vicari, Zlotnik: "Mouse NK1.1+ T cells: a new family of T cells." in: Immunology today, Vol. 17, Issue 2, pp. 71-6, (1996) (PubMed).

Atsuta, Nishimura, Nakamura, Emoto, Iwatsuki, Yoshikai: "Diversity of V gamma gene segments rearranged to the J gamma 4 gene in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 2, pp. 676-84, (1995) (PubMed).

Davenport, Kumar, Bennett: "Rapid rejection of H2k and H2k/b bone marrow cell grafts by CD8+ T cells and NK cells in irradiated mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 8, pp. 3742-9, (1995) (PubMed).

Bendelac, Killeen, Littman, Schwartz: "A subset of CD4+ thymocytes selected by MHC class I molecules." in: Science (New York, N.Y.), Vol. 263, Issue 5154, pp. 1774-8, (1994) (PubMed).

Ohno, Ono, Hirayama, Shimada, Saito: "Preferential usage of the Fc receptor gamma chain in the T cell antigen receptor complex by gamma/delta T cells localized in epithelia." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 365-9, (1994) (PubMed).

Saint-Ruf, Ungewiss, Groettrup, Bruno, Fehling, von Boehmer: "Analysis and expression of a cloned pre-T cell receptor gene." in: Science (New York, N.Y.), Vol. 266, Issue 5188, pp. 1208-12, (1994) (PubMed).

Groettrup, von Boehmer: "T cell receptor beta chain dimers on immature thymocytes from normal mice." in: European journal of immunology, Vol. 23, Issue 6, pp. 1393-6, (1993) (PubMed).

Skeen, Ziegler: "Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection." in: The Journal of experimental medicine, Vol. 178, Issue 3, pp. 971-84, (1993) (PubMed).

Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, (1991) (PubMed).

Bonneville, Itohara, Krecko, Mombaerts, Ishida, Katsuki, Berns, Farr, Janeway, Tonegawa: "Transgenic mice demonstrate that epithelial homing of gamma/delta T cells is determined by cell lineages independent of T cell receptor specificity." in: The Journal of experimental medicine, Vol. 171, Issue 4, pp. 1015-26, (1990) (PubMed).

Gascoigne: "Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3." in: The Journal of biological chemistry, Vol. 265, Issue 16, pp. 9296-301, (1990) (PubMed).

Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, (1989) (PubMed).

Details zu TCR beta

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Hintergrund: The H57-597 antibody reacts with a common epitope of the beta chain of the T-cell Receptor (TCR) complex on alphabeta TCR-expressing thymocytes and peripheral T lymphocytes and NK1.1+ thymocytes and NK-T cells of all mouse strains tested. It does not react with gammadelta TCR-bearing T cells. In the fetal and adult thymus, the TCR beta-chain may form homodimers or pair with the pre-TCR alpha-chain on the surface of immature thymocytes before expression of the TCR alpha-chain. Plate-bound or soluble H57-597 antibody activates alphabeta TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.