TEK Antikörper (pTyr1102) (Alexa Fluor 647)

Produktnummer ABIN1177219

Produktdetails anti-TEK Antikörper (Alexa Fluor 647)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Bindungsspezifität: pTyr1102

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TEK Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human TIE2 Peptide

Klon: K93-754

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-TEK Antikörper (Alexa Fluor 647) (ABIN1177219)

Audero, Cascone, Maniero, Napione, Arese, Lanfrancone, Bussolino: "Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cells." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 13224-33, (2004) (PubMed).

DeBusk, Hallahan, Lin: "Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway." in: Experimental cell research, Vol. 298, Issue 1, pp. 167-77, (2004) (PubMed).

Peters, Kontos, Lin, Wong, Rao, Huang, Dewhirst, Sankar: "Functional significance of Tie2 signaling in the adult vasculature." in: Recent progress in hormone research, Vol. 59, pp. 51-71, (2004) (PubMed).

Jones, Iljin, Dumont, Alitalo: "Tie receptors: new modulators of angiogenic and lymphangiogenic responses." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 257-67, (2001) (PubMed).

Murray, Padrique, Pinko, McTigue: "Mechanistic effects of autophosphorylation on receptor tyrosine kinase catalysis: enzymatic characterization of Tie2 and phospho-Tie2." in: Biochemistry, Vol. 40, Issue 34, pp. 10243-53, (2001) (PubMed).

Edgell, McDonald, Graham: "Permanent cell line expressing human factor VIII-related antigen established by hybridization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 12, pp. 3734-7, (1983) (PubMed).

Details zu TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Andere Bezeichnung: TIE2 (TEK Produkte)

Hintergrund: TIE2 (Tyrosine kinase with Immunoglobulin-like and EGF-like domains 2), also known as TEK (Tunical Endothelial Kinase), is an endothelial and hematopoietic cell-specific receptor tyrosine kinase (RTK) that is critical to the development and maintenance of the vasculature and highly conserved among vertebrate species. The angiopoietins are ligands of TIE2, and the abnormal vascular growth that often occurs in solid tumors is a result of disruptions in the coordinated actions of the angiopoietins, TIE2, and the closely related TIE1 RTK. Upon activation by angiopoietins, TIE2 autophosphorylates at least 2 tyrosines in its protein kinase domain and at least 3 tyrosines in its C-terminal tail. Phosphorylation at tyrosine 1102 (Y1102) leads to activation of the Akt signal transduction pathway, enhancing endothelial cell survival. The phosphorylated Y1102 also recruits and activates the src homology 2 domain-containing adaptor proteins Grb2 and ShcA, affecting endothelial cell adhesion and motility. The K93-754 monoclonal antibody recognizes the phosphorylated Y1102 in the C-terminal tail of activated TIE2. The orthologous phosphorylation site in mouse TIE2 is Y1100.
Synonyms: TEK, VMCM, CD202b

Pathways: RTK Signalweg, Growth Factor Binding